Fig. 2.

Quantities of bacterial species and C. albicans grown as monospecies and polymicrobial biofilms. Standardized numbers of the four species were added to 24-well cell culture plates for monospecies (a) and polymicrobial biofilm (b). The cultures were incubated for 24 and 48 h. At the end of each time point, bacterial viability was checked by plating a small aliquot on brucella blood agar. Bacterial quantities (cells/ml) were determined by using qPCR SYBR Green assay. The results shown are median values from three independent experiments. Abbreviations: Ca = C. albicans, Aa = A. actinomycetemcomitans, Sm = S. mutans, Sg = S. gordonii