Fig. 2.

EV-encapsulated miR-101 from HUCMSCs can be delivered into the HTR-8/SVneo cells. a Expression of miR-101 in placental tissues in patients with PE compared to healthy donors measured by RT-qPCR. b Transfection efficiency of HUCMSCs transfected with cy3-miR-101 mimic, miR-101 mimic, or miR-NC determined using RT-qPCR. c Fluorescence result of HTR-8/SVneo cells after being co-cultured with HUCMSCs transfected with miR-NC, cy3-miR-101, or cy3-miR-101 + GW4869 observed under a fluorescence microscope (scale bar = 20 μm). d Fluorescence result of HTR-8/SVneo cells after being co-cultured with EVs which were labeled with PKH67 and secreted from HUCMSCs transfected with cy3-miR-101 mimic, observed under a fluorescence microscope (scale bar = 20 μm). e Expression of miR-101 in HTR-8/SVneo cells after being co-cultured with HUCMSC-miR-NC, HUCMSC-miR-101, HUCMSC-NC-EV, and HUCMSC-miR-101-EV determined by RT-qPCR. *p < 0.05 vs. **p < 0.01