Fig. 4.

EV-encapsulated miR-101 from HUCMSCs promotes proliferation and migration of placental trophoblasts by inhibiting BRD4 expression. a Cell proliferation of HTR-8/SVneo cells in response to miR-101 mimic, miR-101 inhibitor, or their corresponding NCs determined by EDU staining (scale bar = 50 μm). b Cell migration of HTR-8/SVneo cells in response to transfection with miR-101 mimic, miR-101 inhibitor, or their corresponding NCs determined by transwell assay (scale bar = 50 μm). c Cell proliferation of HTR-8/SVneo cells in response to addition of EV released from HUCMSCs transfected with miR-101 mimic determined by EDU staining (scale bar = 50 μm). d Cell migration of HTR-8/SVneo cells in response to addition of EV released from HUCMSCs transfected with miR-101 mimic determined by transwell assay (scale bar = 50 μm). e Cell proliferation in HTR-8/SVneo cells transfected with miR-101 mimic and oe-BRD4 determined by EDU staining (scale bar = 50 μm). f Cell migration in HTR-8/SVneo cells transfected with miR-101 mimic and oe-BRD4 determined by transwell assay (scale bar = 50 μm). *p < 0.05 vs. **p < 0.01. M.NC, negative control mimic; M.miR-101, microRNA-101 mimic; I.NC, negative control inhibitor; I.miR-101, miRNA-101 inhibitor; BRD4-OE, overexpressed bromodomain-containing protein 4 plasmid