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. 2020 Apr 15;20(1):346–356. doi: 10.3892/ol.2020.11536

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Copyright: © Jiang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Co-treatment with gemcitabine and sorafenib induces apoptosis and cell cycle arrest in A549 cells. A549 cells were treated with 10 µg/ml gemcitabine and 10 µM sorafenib, either alone or in combination for 48 h. (A) Cell cycle distribution was determined by flow cytometry analysis. (B) The quantification of cells in different phases of the cell cycle is presented in bar charts. (C) The percentage of apoptotic cells was determined by flow cytometry analysis. PI, propidium iodide. (D) The percentage of A549 cells positive for Annexin V staining was presented in bar charts. *P<0.05.