Figure 6.

Combination treatment with gemcitabine and sorafenib induces EMT inhibition of A549 cells. A549 cells were treated with a combination of 10 µg/ml gemcitabine and 10 µM sorafenib for 48 h. (A) Protein expression levels of EMT-associated markers N-cadherin, E-cadherin and Twist-1 were assessed by western blot analysis. (B-D) Quantitative analysis of (B) E-cadherin, (C) N-cadherin and (D) Twist-1. (E-G) mRNA expression levels of (E) E-cadherin, (F) N-cadherin and (G) Twist-1. *P<0.05, **P<0.01. EMT, epithelial-to-mesenchymal transition.