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. 2020 Jun 5;11:663. doi: 10.3389/fpls.2020.00663

FIGURE 5.

FIGURE 5

Time course analysis of Arabidopsis under + N, –N and recovery conditions by Raman spectroscopy. (A) Three-week-old seedlings of WT (Col-0) were transferred into +N or –N hydroponic medium. Leaf No. 4 samples were measured by Raman spectroscopy at various time points after transfer to –N medium. n = 10 (biologically independent experiments). (B) Arabidopsis plants grown under –N for 3 days were transferred into +N medium. Plants samples (R) at 0 day were same with samples grown for 3 days under –N medium. Samples were taken at various time points for 4 days. R; plants in the recovery +N medium, Scale bar, 0.5 cm (A,B). a.u; arbitrary unit. n = 10 (biologically independent experiments). (C) Nitrate content of leaf No. 4 was analyzed in +N or recovery plants (R). Data are mean values, n = 12 (biologically independent experiments) and individual data points are shown as overlays. Asterisks indicate statistically significant difference compared with +N. *P < 0.05, **P < 0.01, and ***P < 0.001; two-tailed t-test. FW; fresh weight. Supplementary Table S2 shows P values. (D) ORE1 transcript levels were analyzed by qRT-PCR in samples (leaf #4) of +N or recovery plants (R). Data are mean values, n = 5 (biologically independent experiments) and individual data points are shown as overlays. Asterisks indicate statistically significant difference compared with +N. *P < 0.05, **P < 0.01, and ***P < 0.001; two-tailed t-test. Supplementary Tables S1, S6 show primer set and P values, respectively.