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. 2020 Jun 5;11:663. doi: 10.3389/fpls.2020.00663

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Copyright © 2020 Huang, Singh, Park, Chua, Ram and Park.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Time course analysis of leafy vegetables, Pak Choi and Choy Sum under + N, –N and recovery conditions by Raman spectroscopy. (A,B) Three-week old seedlings of two-week old seedlings of Pak Choi (Brassica rapa chinensis) and Choy Sum (Brassica rapa var. parachinensis) were transferred into +N or –N hydroponic medium. Leaf No. 4 samples were measured by Raman spectroscopy at various time points after transfer to –N medium. n = 10 (biologically independent experiments). (C,D) Pak Choi and Choy Sum plants grown under –N for 3 days were transferred into +N medium. Plants samples (R) at 0 day were same with samples grown for 3 days under –N medium. Samples were taken at various time points for 3 days. n = 5 (biologically independent experiments). R; recovery plant, Scale bar, 0.5 cm. a.u; arbitrary unit.