Table 1.
Primer sequences used for qPCR
| Gene | Accession number | Sequence (5’-3’) |
|---|---|---|
| Agrp | NM_001271806.1 | F: TGACCAAATCCACCCCCTCC R: TTCCTGTAGCCAGGGCATGA |
| Npy | NM_023456.3 | F: TAACAAGCGAATGGGGCTGT R: TTCAAGCCTTGTTCTGGGGG |
| Pomc | NM_001278581.1 | F: GGCGACGGAAGAGAAAAGAGG R: TGTTCAGTCTCCTGCCTGTCG |
| Cart | NM_013732.7 | F: TGGATGATGCGTCCCATG R: TACTTCTTCTCATAGATCGGAAT |
| Orexin | NM_010410.2 | F: TTCCTTCTACAAAGGTTCCCTGG R: CGTAGAGACGGCAGGAACAC |
| Adipoq | NM_009605.5 | F: TGACGACACCAAAAGGGCTC R: CACAAGTTCCCTTGGGTGGA |
| Lep | NM_008493.3 | F: CACACACGCAGTCGGTATCC R: ACTCAGAATGGGGTGAAGCC |
| Lipe | NM_010719.5 | F: GGGTGACTCTAACGCGACTC R: CCTTTAATGGGTGGGGCTGA |
| Acaca | NM_133360.2 | F: GAGAGGGGTCAAGTCCTTCC R: CTGCTGCCGTCATAAGACAA |
| Fasn | NM_007988.3 | F: TGCTCCCAGCTGCAGGC R: GCCCGGTAGCTCTGGGTGTA |
| Ppara | NM_011144.6 | F: ATGAAGAGGGCTGAGCGTAG R: AAACGCAACGTAGAGTGCTGT |
| Cpt1a | NM_013495.2 | F: CAGCACCTGTACCGCCTCGC R: GCCGTCATCAGCAACCGGCC |
| Ucp1 | NM_009463.3 | F: ACTGCCACACCTCCAGTCATT R: CTTTGCCTCACTCAGGATTGG |
| Sirt3 | NM_022433.2 | F: TTTCTTTCACAACCCCAAGC R: ACAGACCGTGCATGTAGCTG |
| Pgc1a | NM_008904.2 | F: TGCCATTGTTAAGACCGA R: GGTCATTTGGTGACTCTGG |
| Hprt1 | NM_013556.2 | F: CACAGGACTAGAACACCTGC R: GCTGGTGAAAAGGACCTCT |
| Pgk1 | NM_008828.3 | F: CTGACTTTGGACAAGCTGGACG R: GCAGCCTTGATCCTTTGGTTG |
| Atp5e | NM_025983.3 | F: TGGCGACAGGCTGGACTCAG R: GCTGCCCGAAGTCTTCTCAGCG |