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. Author manuscript; available in PMC: 2020 Oct 16.
Published in final edited form as: Oncogene. 2020 Apr 16;39(21):4312–4322. doi: 10.1038/s41388-020-1296-2

Figure 1.

Figure 1.

CRSPR/Cas9-based genome-wide screens in VX-680-treated cells reveal GSG2 (also called Haspin) as the top candidate. (A) The flow chart of CRISPR/Cas9 screening in 293A cells treated with DMSO or VX-680. (B) Ranking of the co-essential genes in VX-680-treated samples compared with those treated with DMSO. The Z score from DrugZ analysis of the CRSPR/Cas9 screening results showed the genes with possible synthetic lethal relationships with VX-680. (C) Normalized sgRNA fold changes of GSG2 in DMSO- and VX-680-treated groups from the original screen sequencing data. (D) The top 6 enriched biological processes analyzed through gene ontology (GO; P < 0.01). Genes used for the analysis were high-confidence candidates from (B).