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. Author manuscript; available in PMC: 2020 Jul 7.
Published in final edited form as: Sci Signal. 2020 Jan 7;13(613):eaax4569. doi: 10.1126/scisignal.aax4569

Fig. 4. Identification of the regions of MRAP2 required for the potentiation of G-protein–dependent GHSR1a signaling.

Fig. 4.

(A) Schematic representation of the different MRAP2 mutants generated (TM= transmembrane domain). (B) Western blot detection of MRAP2 mutants from lysates of transfected CHO cells (n=3 independent experiments). (C to H) Ghrelin-stimulated IP accumulation in CHO cells transfected with GHSR1a and empty vector, MRAP2 or the indicated MRAP2 mutant. Statistical analysis of the differences at each ghrelin concentrations were measured by T-test. Data represent the mean +/− SEM of 3 independent experiments performed in triplicate, ***p<0.001.