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. Author manuscript; available in PMC: 2020 Jul 7.
Published in final edited form as: Sci Signal. 2020 Jan 7;13(613):eaax4569. doi: 10.1126/scisignal.aax4569

Fig. 5. Identification of MRAP2 regions required for the inhibition of β-arrestin recruitment to GHSR1a.

Fig. 5.

(A to F) Kinetics of ghrelin-stimulated β-arrestin recruitment to GHSR1a in CHO cells transfected with GHSR1a-LgBiT, SmlBiT-β-arrestin2 and empty vector, MRAP2 or the indicated MRAP2 mutant. Statistical analysis of the differences at each ghrelin concentrations were measured by T-test. Data represent the mean +/− SEM of 3 independent experiments performed in triplicate. (G) Surface density of GHSR1a measured by fixed-cell ELISA in each of the condition tested for β-arrestin recruitment. Statistical analysis was done using one-way ANOVA. Data represent the mean +/− SEM of 3 independent experiments performed in triplicate. (H) Area under the curve of β-arrestin recruitment normalized to the surface expression of GHSR1a for each condition tested. Statistical analysis was done using one-way ANOVA. Data are shown as mean +/− SEM of the percentage of normalized β-arrestin recruitment in the absence of MRAP2. *p<0.05, **p<0.01, ***p<0.001.