Fig. 7. MRAP2 does not increase ghrelin affinity or Gαq protein activation.

(A) ¹²⁵I-Ghrelin competition binding assay in CHO cells transfected with GHSR1a and empty vector or MRAP2. Non-specific binding from mock transfected cells was subtracted and results were normalized to the maximal binding for each condition. Data shown are the mean +/− SEM of 3 independent experiments performed in triplicate. (B) GTPγS binding assay in membranes prepared from CHO cells transfected with GHSR1a and empty vector or MRAP2. Binding values obtained with membranes from mock transfected cells was subtracted and results were normalized to GHSR1a expression measured by ELISA. Statistical analysis was done using one-way ANOVA. Results shown represent the data attained from 3 independent experiments. Error bar are SEM. ***p<0.001, **p<0.01