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. 2020 Jun 5;11:578. doi: 10.3389/fphys.2020.00578

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Copyright © 2020 San-Millán, Stefanoni, Martinez, Hansen, D’Alessandro and Nemkov.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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High-Energy Phosphates and Purine Metabolism. (A) A pathway map for high-energy phosphate and purine homeostasis is shown. Spontaneous, non-enzymatic reactions are shown in red arrows. Metabolite abundances [y-axis, Peak Area (AU)] are plotted for (B) Creatine homeostasis, (C) Purine salvage, and (D) Purine catabolism. Samples from the Gold (°) and Silver (□) groups are shown Pre and Post exercise test (divided by a dotted line). p-values from a two-tailed paired T-test of comparisons between the Pre and Post time points using combined Gold/Silver group values are shown above the dashed line. p-values from a two-tailed unpaired homoscedastic T-test comparing the Gold and Silver groups at each time point are shown on the respective side of the dashed line. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.