TABLE 1.
Physicochemical parameters of parental and designed peptides.
| Peptide | Sequences | MWa | Net charge | Hydrophobicity | A1b | A2c |
| DLP4 | ATCDLLSPFKVGHAACAAHCIARGKRGGWCDKRAVCNCRK | 4269.05 | 6 | 0.354 | 16 | 16 |
| ID1 | ATADLLSPFKVGHAACAAHCIARGKRGGWADKRAVCNCRK | 4206.93 | 6 | 0.292 | – | – |
| ID2 | ATCDLLSPFKVGHAAAAAHCIARGKRGGWCDKRAVANCRK | 4206.93 | 6 | 0.292 | – | – |
| ID3 | ATCDLLSPFKVGHAACAAHAIARGKRGGWCDKRAVCNARK | 4206.93 | 6 | 0.292 | – | – |
| ID4 | ATCDILSPFKVGHAACAAHCIARGKRGGWCDKRAVCNCRK | 4269.05 | 6 | 0.356 | 32 | ND |
| ID5 | ATCDLISPFKVGHAACAAHCIARGKRGGWCDKRAVCNCRK | 4269.05 | 6 | 0.356 | 16 | ND |
| ID6 | ATCDLLTPFKVGHAACAAHCIARGKRGGWCDKRAVCNCRK | 4283.08 | 6 | 0.361 | 8 | 16 |
| ID7 | ATCDLLSPFKVGHAACAAHCIARGKRSGWCDKRAVCNCRK | 4299.08 | 6 | 0.353 | – | – |
| ID8 | ATCDLLSKFKVGHAACAAHCIARGKRGGWCDKRAVCNCRK | 4300.11 | 7 | 0.311 | 32 | ND |
| ID9 | ATCDLLSPFKVKHAACAAHCIARGKRGGWCDKRAVCNCRK | 4340.17 | 7 | 0.329 | 16 | ND |
| ID10 | ATCDLLSPFKVGHAACAAHCIARGKRGGWCNKRAVCNCRK | 4268.07 | 7 | 0.358 | 16 | ND |
| ID11 | ATCDLLSPFGVGHAACAAHCIARGKRGGWCDKRAVCNCRK | 4197.93 | 5 | 0.379 | 16 | ND |
| ID12 | ATCDLLSPFKVGHAACAAHCIARGNRGGWCDKRAVCNCRK | 4254.98 | 5 | 0.364 | – | – |
| ID13 | ATCDLLSPFKVGHAACAAHCIARGKRGGWCDGRAVCNCRK | 4197.93 | 5 | 0.379 | 4 | 4 |
| ID14 | ATCDLLSPFKVGHAACAAHCIARGKRGGWCDKRAVCNCRN | 4254.98 | 5 | 0.364 | – | – |
| ID15 | ATCDLLSPFGVGHAACAAHCIARGNRGGWCDKRAVCNCRK | 4183.86 | 4 | 0.388 | – | – |
| ID16 | ATCDLLSPFGVGHAACAAHCIARGKRGGWCDGRAVCNCRK | 4126.81 | 4 | 0.403 | 8 | 16 |
| ID17 | ATCDLLSPFGVGHAACAAHCIARGKRGGWCDKRAVCNCRN | 4183.86 | 4 | 0.388 | 16 | 64 |
| ID18 | ATCDLLSPFKVGHAACAAHCIARGNRGGWCDGRAVCNCRK | 4183.86 | 4 | 0.388 | 16 | ND |
| ID19 | ATCDLLSPFKVGHAACAAHCIARGNRGGWCDKRAVCNCRN | 4240.91 | 4 | 0.373 | – | – |
| ID20 | ATCDLLSPFKVGHAACAAHCIARGKRGGWCDGRAVCNCRN | 4183.86 | 4 | 0.388 | 16 | ND |
| ID21 | ATCDLLSPFGVGHAACAAHCIARGNRGGWCDGRAVCNCRK | 4112.74 | 3 | 0.413 | – | – |
| ID22 | ATCDLLSPFKVGHAACAAHCIARGNRGGWCDGRAVCNCRN | 4169.79 | 3 | 0.398 | – | – |
| ID23 | ATCDLLSPFKVGHAACAAHCIARGKRGGWCDDRAVCNCRN | 4241.89 | 3 | 0.369 | – | – |
| ID24 | ATCDLLSKFKVKHAACAAHCIARGKRGGWCDKRAVCNCRK | 4371.23 | 8 | 0.286 | – | – |
| ID25 | ATCDLLSPFKVKHAACAAHCIARGKRGGWCNKRAVCNCRK | 4339.19 | 8 | 0.333 | – | – |
| ID26 | ATCDLLSKFKVGHAACAAHCIARGKRGGWCNKRAVCNCRK | 4299.12 | 8 | 0.315 | – | – |
| ID27 | ATCDLLSPFKVGHAACALHCIARGKRGGWCDKRAVCNCRK | 4311.13 | 6 | 0.388 | 8 | 8 |
| ID28 | ATCDLLSPFKVGHAACAVHCIARGKRGGWCDKRAVCNCRK | 4297.1 | 6 | 0.377 | 8 | 16 |
| ID29 | ATCDLLSPFKVGHAACAAHCLARGKRGGWCDKRAVCNCRK | 4269.05 | 6 | 0.351 | 64 | 128 |
| ID30 | ATCDLLSPFKVGHAACAAHCIARGKRGGWCDKRAICNCRK | 4283.08 | 6 | 0.368 | 16 | 32 |
Changed amino acids are shown in bold and underlined. aMolecular weight (Da). b Activities (MICs, μg/mL) of peptides were tested against S. aureus ATCC 43300; inactive peptides are indicated by “–,” tested by drop-diffusion assay. c Activities (MICs, μg/mL) of peptides were tested against S. aureus CVCC 546; inactive peptides are indicated by “–,” tested by drop-diffusion assay; ND: not determined.