Figure 3.
The viabilities of (A) H157; (B) MCF-7; (C) PC-3; (D) U251MG cells treated with Dermaseptin-PP at gradient concentrations from 10−4 to 10−9 M determined by MTT assays. Vehicle control: cells treated with 1% PBS; Medium control: cells treated with pre-warmed serum-free medium. Values are mean ± SEM of three independent experiments. Data was analyzed with Dunnett's multiple comparisons of one-way ANOVA using GraphPad Prism. The significance was expressed as p-values: ****P < 0.0001 vs. vehicle control. The percentage of LDH release from (E) HMEC-1 cells and (F) H157 cells treated by Dermaseptin-PP (10−4-10−9 M). Values are mean ± SEM of three independent experiments. Data was analyzed with Dunnett's multiple comparisons of one-way ANOVA using GraphPad Prism. “****” indicates p < 0.0001 and “**” represents p < 0.01 compared with negative control (1% PBS).