Figure 4.
(A) The morphology changes of H157 cells after treatment with gradient Dermaseptin-PP (10−4 to 10−9 M) for 24 h. (B) The morphology changes of H157 cells after Dermaseptin-PP (10−4 M) treatment for different time (30 min, 1, 2, 4, 8, 16, 24 h). The cell membrane and the nucleus were dyed red and blue respectively. The images were observed under the laser confocal microscope by a magnification factor × 60. (C) The representative images of H157 cells treated with different concentrations of Dermaseptin-PP from flow cytometry analysis. (D) The apoptosis rate of H157 cells treated by gradient Dermaseptin-PP (10−4 to 10−9 M) was quantified based on flow cytometry analysis. Dermaseptin-PP could promote apoptosis of H157 cells from concentration of 5 × 10−6 M. All data was represented as the mean ± SEM of three independent experiments. The significance of group differences was determined by P-value. “****” represents P < 0.0001 vs. blank control group (cells without Dermaseptin-PP treatment).