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Thermodynamic Parameters for Binding of Cytc to CL NDs from CD Data at 25 °C and pH 8.
| Variant | Kd(app) (μM ND)a | n |
|---|---|---|
| Monomer-ND | 2.3 ± 0.1 | 0.9 ±0.3 |
| Dimer-ND | 0.3 ± 0.1 | 1.2 ± 0.4 |
The experimental concentration of cytc was 10 μM based on the heme absorbance (i.e., 10 μM monomer or 5 μM DSD). The error corresponds to the standard error in the fit of the parameter. We use apparent dissociation constant, Kd(app), because we have not corrected ND concentration for ND bound to cytc.
