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Detection of YMV-CP in regenerated plants. (A) RT-PCR of plants regenerated from cryo-treated buds using YMV-CP specific primers; (B) Yam ubiquitin; (C) RT-PCR of plants regenerated from non-cryotreated buds using YMV-CP specific primers; (D) Yam ubiquitin; M – DNA marker; Cs – YMV-infected stock plant; Mx – PCR master mix; 1–20 – plants regenerated from cryo-treated axillary buds; 1–10 – YMV-infected plants regenerated from non-cryotreated axillary buds.
