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. 2020 Jun 5;11:1110. doi: 10.3389/fimmu.2020.01110

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Copyright © 2020 Martin, Hyrlov, Elkjaer, Thygesen, Wlodarczyk, Elbaek, Aboo, Okarmus, Benedikz, Reynolds, Hegedus, Stensballe, Svenningsen, Owens and Illes.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of miR-146a in brain resident cells and microglia in miR-146a KO mice. (A) Using MACS, microglia were sorted by anti-CD11b, OPCs by anti-PDGFRα, immature oligodendrocytes by anti-O4, astrocytes by anti-ACSA-2, and neurons by negative selection. Purity of each population is indicated. Relative expression of miR-146a was determined by qPCR and expression levels are presented relative to geometric mean of U6snRNA and sno135 levels. Each sample is a pool of 3–5 brains. Statistics: *p < 0.05, ***p < 0.001, n = 5–8 in each group, one-way ANOVA followed by Tukey's multiple comparisons test, mean ± SD. (B) The number of microglia was analyzed by flow cytometry, and microglia were defined as CD11b⁺/CD45^low cells. n = 6–7 in each group, parametric students t-test, mean ±SD.