TABLE 1.

General linear Model (GLM) analysis on the selected microbial genes.

KEGG ID gene	General function	Protein	GITa location of possible impact	Mean abundance LOW	Mean abundance HIGH	F-value	P-value
K01206	Fucose sensing	Alpha-L-fucosidase	Intestine	0.012	0.010	1.187	0.325
K01654	Mucus interaction	N-acetylneuraminate synthase	Rumen and intestine	0.0020	0.0021	3.425	0.013
K01818	Fucose sensing	L-fucose isomerase	Intestine	0.014	0.015	0.92	0.458
K01835	Biofilm formation	Phosphoglucomutase	Rumen and intestine	0.005	0.001	3.429	0.013
K01840	Biofilm formation	Phosphomannomutase	Rumen and intestine	0.277	0.250	4.989	0.001
K01993	Secretion system	HlyD family secretion protein	Rumen and intestine	0.0137	0.0145	2.806	0.032
K02005	Secretion system	HlyD family secretion protein	Rumen and intestine	0.118	0.099	2.101	0.09
K02283	Pilus	Pilus assembly protein CpaF	Rumen and intestine	0.0068	0.0071	4.431	0.003
K02377	Fucose sensing	GDP-L-fucose synthase	Intestine	0.034	0.034	1.181	0.327
K02390	Flagella	Flagellar hook protein FlgE	Rumen and intestine	0.001	0.001	1.91	0.119
K02392	Flagella	Flagellar basal-body rod protein FlgG	Rumen and intestine	0.002	0.003	1.533	0.203
K02396	Flagella	Flagellar hook-associated protein 1 FlgK	Rumen and intestine	0.001	0.001	1.436	0.232
K02400	Flagella	Flagellar biosynthesis protein FlhA	Rumen and intestine	0.006	0.008	0.886	0.477
K02406	Flagella	Flagellin	Rumen and intestine	0.011	0.013	1.356	0.259
K02407	Flagella	Flagellar hook-associated protein 2	Rumen and intestine	0.005	0.007	0.468	0.759
K02410	Flagella	Flagellar motor switch protein FliG	Rumen and intestine	0.001	0.002	0.868	0.488
K02412	Flagella	Flagellum-specific ATP synthase	Rumen and intestine	0.001	0.002	4.559	0.003
K02429	Fucose sensing	MFS transporter, FHS family, L-fucose permease	Intestine	0.031	0.025	0.864	0.49
K02454	Secretion system	General secretion pathway protein E	Rumen and intestine	0.005	0.006	11.788	0.001
K02652	Pilus	Type IV pilus assembly protein PilB	Rumen and intestine	0.010	0.015	8.995	0.001
K02653	Pilus	Type IV pilus assembly protein PilC	Rumen and intestine	0.004	0.006	10.751	0.001
K02662	Pilus	Type IV pilus assembly protein PilM	Rumen and intestine	0.002	0.002	2.048	0.098
K02666	Pilus	Type IV pilus assembly protein PilQ	Rumen and intestine	0.003	0.006	2.113	0.089
K03205	Secretion system	Type IV secretion system protein VirD4	Rumen and intestine	0.007	0.011	1.458	0.225
K06442	Virulence	Putative hemolysin	Intestine	0.0033	0.0031	4.155	0.005
K11068	Virulence	Hemolysin III	Intestine	0.004	0.003	2.069	0.095
K11907	Secretion system	Type VI secretion system protein VasG	Rumen and intestine	0.011	0.013	1.564	0.194

^aGIT: Gastrointestinal tract with two locations identified as rumen or intestine. Year of animal trial and Breed were included as fixed effect in the GLM analysis. Bold: Genes with a relative abundance significantly different (P < 0.05) between Low and High feed efficiency (both FCR and RFI) animal groups.