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. 2020 Jun 12;15(6):e0234468. doi: 10.1371/journal.pone.0234468

Fig 4. Differential scanning fluorimetry (DSF) shows effect of fisetin and other flavonoids on human SUMO1.

Fig 4

Treatment of 2 μM human SUMO1 with 300 μM flavonoid indicated that (A) fisetin, (B) quercetin, and (C) isoliquiritigenin cause unfolding of human SUMO1 leading to a DSF thermogram resembling unfolded protein. (D) In contrast, kaempferol treatment results in little or no effect. (E) Treatment of 2 μM SUMO1Δ15 with increasing concentration of fisetin (from 0.1 μM to 200 μM) results in a dose-dependent change in the DSF thermogram of SUMO1. Experiments were performed with five technical replicates.