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. 2020 Jun 11;5(48):eabc8413. doi: 10.1126/sciimmunol.abc8413

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Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY).

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Fig. 1 — Production and characterization of the RBD of the coronavirus spike antigens. (A) The spike protein on the virion surface engages its cognate receptor via the RBD. (B) RBD of the spike protein is the main human antibody target in SARS-CoV-1. (C) The amino acid sequence corresponding to RBD of the spike protein is poorly conserved between SARS-CoV-2 and common human coronaviruses. (D) Coomassie-stained SDS-PAGE of purified spike RBD antigens from different CoVs. (E) Binding characterization of the spike RBD antigens with immune sera and a monoclonal antibody. SARS-CoV-1 monoclonal antibody (240C), serum from a mouse immunized with VRP expressing SARS-CoV-2 or SARS-CoV-1 spike protein, serum from a rabbit immunized with SARS-CoV-1 spike protein and an archived human sample collected before SARS-COV-2 were tested for binding against RBD spike antigens from SARS-CoV-2, SARS-Co-V-1, HCoVα (NL63) and HCoVβ (HKU-1).