Figure 5.

CRISPR/Cas9‐mediated mutation of CGP1. (a) Schematic representation of the CGP1 gene and the two target sites used for CRISPR/Cas9‐mediated mutagenesis. CGP1‐F and CGP1‐R are the forward and reverse primers used for the amplification of the genomic fragment. (b) Phenotypes of CGP1 knockout lines P15 and P22. (c) PCR amplification of the DNA fragment containing two target sites in five individual P15 and P22 T1 plants. (d, e) Sequencing of the PCR products where deletions are shown as red dashed lines.