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. 2020 Jan 21;18(7):1573–1584. doi: 10.1111/pbi.13323

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© 2019 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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CGP1a interacts with GoPGF in the nucleus. (a) Yeast two‐hybrid assays showing interaction between PGF2a and GoPGF but not between PGF2d and GoPGF. Yeast growth and X‐Gal staining indicate interaction. (b) BiFC assays showing interaction between PGF2a and GoPGF in nuclei of tobacco epidermal cells. PGF2a‐cYFP and GoPGF‐nYFP were co‐expressed in tobacco leaves for the interaction assay. BES1‐cYFP and BIN2‐nYFP were used as positive control; bar = 10 μm. (c) Co‐IP assays of CGP1a and GoPGF. CGP1a‐GFP, GoPGF‐Flag or CGP1a‐GFP/GoPGF‐Flag were expressed in tobacco leaves. The Co‐IP experiment was performed with anti‐GFP affinity gel resin. The isolated proteins were analysed by immunoblotting with anti‐GFP antibody for the detection of CGP1a and with anti‐Flag antibody for the detection of GoPGF.