Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (S. cerevisiae) | AJY# | This paper and previous literatures. | N.A. | Detailed information of strain genotypes is provided inSupplementary file 2. See plasmid and strain construction below for more details. |
| Recombinant DNA reagents | pAJ# | This paper or obtained by requests to other labs | N.A. | Detailed information of the recombinant DNA reagents (plasmids) used in this study is provided in Supplementary file 3. See plasmid and strain construction below for more details. |
| Chemical compound, drug | Benomyl | Millipore Sigma | 45339 | Both of the benomyl powders have been used in spotting and plate reader assays. Please refer to materials and methods for concentration of Benomyl. See benomyl sensitivity assay mentioned below for more details. |
| Chemical compound, drug | Benomyl | Millipore Sigma | 381586 | |
| Chemical compound, drug | Nocodazole | Fisher Scientific | AC358240100 | Used to disrupt spindle and create unattached kinetochores. Please see below for concentration of Nocodazole. |
| Chemical compound, drug | Propidium Iodide (PI) | Millipore Sigma | P4864 | Used in flow cytometry. Please refer to flow cytometry section below for concentration of PI, |
| Chemical compound, drug | Rapamycin | Fisher Scientific | NC9362949 | Used in phosphodomain tethering assays. Please see flow cytometry section for concentration of Rapamycin. |
| Chemical compound, drug | RNase | Fisher Scientific | SIG10109169001 | Used in flow cytometry. Please refer to flow cytometry section for concentration of RNase. |
| Software/algorithm | Graphpad Prism | Graphpad software Inc | Prism Version 8 | Used for making plots and performing statistical analyses |
| Software/algorithm | ImageJ (Fiji) | https://imagej.net/Fiji/Downloads | Used for Spc105, phosphodomain tethering, Bub3, Bub1, Mad1 and Sgo1 intensity measurement. | |
| Software/algorithm | Matlab | Mathworks | For colony counting, we used Colony counter | |
| Software/algorithm | Metamorph | Molecular devices | Used for imaging involving microscopy assays. Please refer to Materials and methods for details of imaging | |
| Software/algorithm | Adobe Illustrator | Adobe creative cloud | Version 2019 | Used to assemble the data and to prepare the figures |
| Other | Gene and protein information resources for S. cerevisiae | Saccharomyces genome database (https://www.yeastgenome.org) | N.A. | Used for recombinant DNA reagents and S. cerevisiae strain construction. |