Video 5. Replacement of NaCl with choline Cl or KCl does not affect the G2-mediated Ca²⁺ influx.

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CHO cells were co-transfected with RUSH-G2 and GCaMP6f for 24 hr prior to imaging. On the day of the experiment cells were treated with 80 µM biotin and incubated in media containing 150 mM Na⁺ (130 mM NaCl), 85 mM Na⁺ and 65 mM choline⁺, or 85 mM Na⁺ and 65 mM K⁺. The G2-mediated Ca²⁺ influx was unaffected by reduced Na⁺. 3 different fields are shown for each condition. Cells were imaged every 5 min from 0.5 to 12 hr post-biotin. Scale bar = 100 µm.