Figure 4.

C‐type lectin‐like receptor 2 (CLEC‐2) deficiency results in absence of alveolar duct myofibroblasts (adMYFs), lung mesothelial cell hyperproliferation, and abnormal differentiation. (A) Immunostaining of α‐smooth muscle actin (α‐SMA) (green) and DAPI (blue) in E16.5/E17.5 distal lung. Arrowheads: bronchial smooth muscle cells, which are not adMYFs. (B) Whole‐mount immunohistochemistry of Wt1 (magenta) and Pdpn (green) in E17.5 lung mesothelial cells (luMCs). Arrowheads: Wt1‐negative luMCs. (C) Quantification of Wt1‐negative rate in luMCs at E15.5, E16.5, and E17.5; mean ± SD, n = 5‐3 each. *P = .0001; **P = .0074, Tukey's test. Scale bars: 25 μm (A, B). Modified from Tsukiji et al, ³⁸ Figures 2 and 3