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. Author manuscript; available in PMC: 2021 May 5.
Published in final edited form as: Immunobiology. 2020 May 5;225(3):151952. doi: 10.1016/j.imbio.2020.151952

Fig. 2. Surface markers before normalization.

Fig. 2.

Cells were gated according to nuclear fluorescence into three groups: one nucleus (1N; black bars), two nuclei (2N; gray), and three or more nuclei (≥3N; white). Median fluorescence intensity (MFI) of all surface markers increases in binucleated macrophages (2N) and MGC (≥3N), which may lead to the conclusion that marker expression is increased in these cells. However, the increase in fluorescence may simply be a result of larger cell size, with actual membrane density distribution of markers remaining unchanged. This demonstrates the importance of data normalization.