(a) Differential gene expression by glia (#1-9) and
neurons (#10-45). Because of the integration of six stages, early-expressed
TFs and spatially-restricted genes amenable to cellular differentiation were
identified. For neuronal clusters, fast neurotransmitter specificity is
shown to the right. Relative diameter of the solid circles
for each cluster is scaled to the fraction of cells that expresses a
specific gene. Color coding and numbering at the top
correspond to those in Figure 1a.
(b) Dot plot representation of differential TF expression
in 45 ectoderm-derived cell groups in the hypothalamus.
(b1,b2) Subclass-specific TFs
recapitulate the UMAP positions of neuronal (b1) and
tanycyte (b2) subtypes. (c) Integrated
molecular/anatomical annotation of hypothalamic with their specific
assignment to hypothalamic areas. Abbreviations: ARC-Agrp,
arcuate nucleus-agouti-related peptide+ neurons; ARC-Sst, arcuate
nucleus-somatostatin+ neurons; ARC-TIDA, arcuate
nucleus-tuberoinfundibular dopamine neurons; DMH, dorsomedial hypothalamus;
Gal, galanin;
Ghrh/Vacht, growth hormone-releasing
hormone/vesicular acetylcholine transporter+ neurons; LH, lateral
hypothalamus; LH-Lhx9, lateral hypothalamus-LIM homeobox
9+ cluster; Meis2, meis homeobox 2; MM, ;
MM-Lhx9, mammillary nucleus-LIM homeobox 9+
neurons; Pomc, proopiomelanocortin; PH, posterior hypothalamus; PMM,
premamillary nucleus; PVN, paraventricular nucleus; SCN, suprachiasmatic
nucleus; Tbr1, T-box brain transcription factor 1; VMH,
ventromedial hypothalamic nucleus.