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. Author manuscript; available in PMC: 2020 Aug 17.
Published in final edited form as: Nat Neurosci. 2020 Feb 17;23(3):363–374. doi: 10.1038/s41593-019-0581-2

Extended Data Fig. 6. Characterisation of OPC GCaMP reporter lines.

Extended Data Fig. 6

a) Example images of individual olig1:GCaMP-CAAX labelled OPCs in axo-dendritic areas of the zebrafish spinal cord at 4 dpf. The absence of nascent ensheathments indicates that these cells are not early differentiating oligodendrocytes (n=9 independent experiments). Scale bar: 10 μm.

b) Dorsal views of Tg(olig1:GCaMP6m), Tg(mbp:KillerRed) transgenic zebrafish at 4 dpf to label OPCs and differentiated oligodendrocytes. Dotted box indicates position of zoom-ins in bottom row (n=3 animals in 1 experiment). Scale bars: 50 μm (top) and 20 μm (bottom).

c) Quantification of single and double-positive cells from images as shown in b.

d) ΔF/F0 GCaMP transients of individual cells in two Tg(olig1:GCaMP6m) zebrafish. Green traces depict cells in axo-dendritic areas, grey traces depict cells in neuron-rich areas (total of 8 animals in 8 experiments).