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. Author manuscript; available in PMC: 2020 Aug 17.
Published in final edited form as: Nat Neurosci. 2020 Feb 17;23(3):363–374. doi: 10.1038/s41593-019-0581-2

Extended Data Fig. 1. Characterisation of OPCs in the zebrafish spinal cord.

Extended Data Fig. 1

a) Confocal image of a Tg(olig1:memEYFP), Tg(olig1:nls-mApple) zebrafish at the level of the spinal cord at 21 dpf (example of 3 animals from 1 experiment). Scale bar: 50 μm.

b) Cross-sectional view of the spinal cord showing the distribution of myelin in Tg(mbp:EGFP-CAAX) at 7 dpf (example of 12 animals from 4 experiments). Scale bar: 10 μm.

c) Cross-sectional view of the spinal cord showing the distribution of pre- and postsynapses (Tg(elavl3:synaptophysin-RFP), anti-mCherry, anti-gephyrin) at 7 dpf (example of 12 animals from 4 experiments). Scale bar: 10 μm.

d) Confocal images of Tg(mbp:nls-EGFP), Tg(olig1:nls-mApple) transgenic animals between 4 and 28 days post fertilization (n numbers as in panel e). Scale bar: 20 μm.

e) Cell numbers of OPCs (olig1: nls-mApple-pos., mbp:nls-EGFP-neg.) and myelinating oligodendrocytes (mbp:nls-EGFP-pos.) in the spinal cord. Data are expressed as mean cells/field ± SD at 3/5/7/10/13/16/20/24/28 dpf (OPCs: 29.6±6.4 vs. 28.1±5.2 vs. 22.3±4.3 vs.19.3±4.5 vs. 21.7±5.4 vs. 22.6±4.2 vs. 25.3±4.6 vs. 27.3±5.1 vs. 32.0±5.9; mOLs: 7.3±5.7 vs. 31.1±5.5 vs. 44.7±6.0 vs. 56.6±6.9 vs. 62.6±7.2 vs. 61.5±11.6 vs. 74.1±9.4 vs. 75.7±13.3 vs. 95.2±13.4; n animals / experiments=17/2, 15/3, 15/3, 16/3, 17/2, 17/2, 20/3, 12/3, 13/3).

f) Example images of individual OPCs labelled showing a range of morphologies. The soma can be localised within axo-dendritic (top) or neuron-rich areas (middle, bottom). The process network of an individual cell can be restricted to one side of the spinal cord (top and middle cells), but it can also reach to both sides of the spinal cord (bottom cell) (n numbers as in panel g). Scale bar: 10 μm.

g) OPC morphometry using three-dimensional process tracing and creation of a volume hull around the reconstructed filaments (n=228 cells from 56 animals between 3 and 16 dpf in 24 experiments). Scale bar: 10 μm.