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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Hum Mutat. 2020 Apr 1;41(7):1298–1307. doi: 10.1002/humu.24014

Figure 1. Immunoblotting analysis of wild-type ABCA3 and mutant proteins.

Figure 1.

Total protein was extracted from A549 cells transduced with adenovirus expressing WT or ABCA3 variant constructs encoding p.Asn140His, p.Phe1203del, p.Ser1262Gly, p.Val1399Met, p.Leu101Pro, or p.Glu292Val. ABCA3-GFP protein was detected using anti-GFP antibody and normalized to β-actin. Except for the previously characterized type I trafficking mutant p.Leu101Pro, all mutant ABCA3 proteins demonstrated immunoblotting patterns similar to WT-ABCA3.