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. 2020 Jun 12;10:9559. doi: 10.1038/s41598-020-66447-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Establishment of hopless^wz18 zebrafish mutant. (A) A pair of gRNAs (indicated by scissors) were designed to target the upstream and downstream genomic regions flanking the zebrafish hop cassette. (B) PCR amplification of the genomic hop-cassette site demonstrate a single truncated product in the mutant genome. (C) Amplification of PAC1-hop region in the zebrafish PAC1 mRNA shows loss of the hop isoform in cDNA produced from 6 dpf larvae. Plus sign represents wild-type allele; minus sign indicates hopless allele. Full length source electorophoresis gel images are provided in Supplementary Fig. 1.