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. 2020 Apr 18;37:100985. doi: 10.1016/j.molmet.2020.100985

Figure 2.

Figure 2

Pomc restoration in GABAergic-POMC neurons improves energy balance and glycemia. (A, H) Average of daily food intake from the 6th to 8th and from the 12th to 14th weeks of age (before (PRE) and after (POST) treatment, respectively) of mice treated i. p. with tamoxifen (TAM) at P60. N = 9–14. (B, I) Body weight curves of mice treated with TAM at P60. Graphs on the right show comparisons of body weights before (PRE, 7 weeks old) and after (POST, 16 weeks old) TAM treatment. N = 9–14. (C, J) Fast induced hyperphagia of mice previously treated with TAM at P60. Bars correspond to averages of 24-hour refeeding data expressed as a percentage of prefasting food intake. The degree of hyperphagia can be estimated above the dashed lines. N = 9–14. (D, K) Basal glycemia measured before (PRE, 7 weeks old) and after (POST, 14 weeks old) TAM treatment. N = 5–9. (E-G and L-N) Gonadal and inguinal fat pad weights and liver weights of mice treated with TAM at P60. N = 4–10. (O, R) Average of daily food intake (from the 12th to 14th weeks of age) of mice treated i. p. with TAM at P25. N = 4–11. (P–S) Body weight curves of mice treated with TAM at P25. Graphs on the right show comparisons of body weights before (PRE, 3 weeks old) and after (POST, 16 weeks old) TAM treatment. N = 4–12. (Q, T) Basal glycemia measured at the 14th weeks of age of mice treated with TAM at P25. N = 3–9. (A–G) and (O–Q) correspond to females and (H–N) and (R–T) correspond to males. In all cases, error bars: ±SEM. (A, B, D, H, I, K, P, S) RMA: ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001; #P < 0.05, ##P < 0.01, and ###P < 0.001: pairwise comparisons within the same group, before (PRE) versus after (POST) Pomc restoration (Bonferroni). (C, E–G, J, L–N, O, Q, R, T) OWA: ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 (Bonferroni).