DMH-NPY expression is normalized after POMC expression restricted to GABAergic neurons. (A, C) Coronal brain sections of mice subjected to in situ hybridization, showing Npy mRNA (purple) in the dorsomedial hypothalamic nucleus (DMH) and arcuate nucleus (Arc) of the hypothalamus. 3V, third ventricle. ME: median eminence. Magnification bars: 200 μm. (B, D) Average of DMH-Npy and Arc-Npy neurons per hemisection for each genotype. Error bars: ±SEM. OWA: ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 (Bonferroni). N = 3–4.