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. 2020 Apr 18;37:100985. doi: 10.1016/j.molmet.2020.100985

Figure 6.

Figure 6

Arcuate POMC neurons projecting to DMH are mainly GABAergic. (A) Representative image of coronal brain sections showing bilateral injection of retrobeads (red) in the DMH of a wild-type female mouse. Magnification bar: 200 μm. (B) Representative fluorescent microphotograph of a coronal brain section at −1.58 mm from bregma, subjected to POMC immunostaining (blue) and in situ hybridization against Gad1 (green). Red: retrobeads coming from the DMH. Magnification bar: 200 μm. (C–F) Magnified images of the square shown in (B). Arc: arcuate nucleus. 3V: third ventricle. Magnification bar: 50 μm.