Fig. 3.

Model validation using high-throughput phenotypic data for different carbon and nitrogen sources. A) Predicted growth values of A. vinelandii DJ in nitrogen fixation (N₂ ​+ ​Carbon source) or ammonium assimilation (NH₄ ​+ ​Carbon source) conditions using 121 carbon sources (PM1 and PM2 from Biolog Plates). However, only 71 carbon sources are shown in Fig. 3. B) Estimated growth for 75 nitrogen sources, nevertheless just 12 nitrogen compounds are presented in the graph (PM3 from Biolog Plates). Pyruvate was used to constrain the model as the unique carbon source during the nitrogen validation. C) statistical parameters and Matthews correlation coefficient of the predictions for the carbon (first column) and nitrogen sources (second column) using the Biolog Plates information; true positive (TP), true negative (TN), false positive (FP) and false negative (FN). Statistical analysis of the estimations for 38 carbon sources under nitrogen fixation and H₂ consumption conditions (third column). The data used during the simulations were obtained from Wong and Maier (1985).