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. 2020 Jun 4;11:e00133. doi: 10.1016/j.mec.2020.e00133

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© 2020 The Authors. Published by Elsevier B.V. on behalf of International Metabolic Engineering Society.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 2 — Screening ofLactococcus lactissubsp.lactisbiovardiacetylactis strains for amino acids secretion. (A) GFPsensor fluorescence signal (y-axis) when the GFPsensor strain is co-cultivated with different producer strains (x-axis), the higher the GFPsensor fluorescence, the higher the number of GFPsensor cells. (B, C, D) Representative snapshots of co-cultivation of the GFPsensor with three different producer strains by fluoresce microscopy. The three strains are able to grow, but IPLA838 (B) promotes poor growth of the GFPsensor, NCDO176 (C) promotes better growth of the GFPsensor, and the presence of WW4 (D) highly promotes the growth of the GFPsensor. Overlays of the fluorescence-channel and the bright-field are shown.