Table 1.
Contents of essential amino acids in culture supernatants of wild-type strains.
| Sample | Glu | His | Val | Met | Ile | Leu | |
|---|---|---|---|---|---|---|---|
| CDM-casein | B | ND | ND | 0.8 ± 0.1 | ND | ND | 1.1 ± 0.1 |
| SK11 | A | 14.2 ± 0.2 | ND | 1.1 ± 0.1 | ND | 0.7 ± 0.1 | 0.7 ± 0.1 |
| B | 39.7 ± 0.8 | 5.8 ± 0.3 | 1.7 ± 0.1 | ND | 2.2 ± 0.3 | 0.7 ± 0.1 | |
| NZ9000pLP712 | A | 36.6 ± 1.0 | 4.4 ± 0.5 | 5.5 ± 0.5 | 5.2 ± 0.9 | 3.6 ± 0.4 | 2.9 ± 0.2 |
| B | 60.8 ± 0.5 | 9.9 ± 0.2 | 10.1 ± 0.4 | ND | 3.0 ± 0.1 | 10.0 ± 0.2 | |
| MG610 | A | 3.3 ± 0.2 | ND | ND | ND | 0.9 ± 0.1 | ND |
| B | 5.3 ± 0.1 | ND | ND | ND | 0.7 ± 0.1 | ND | |
| Wg2 | A | 15.0 ± 0.2 | 2.6 ± 0.1 | 36.0 ± 0.7 | 6.6 ± 0.4 | 11.7 ± 0.4 | 18.2 ± 0.1 |
| B | 29.3 ± 0.7 | 4.6 ± 0.1 | 67.9 ± 1.3 | 11.7 ± 0.4 | 23.5 ± 0.2 | 35.0 ± 0.2 | |
| NCDO176 | A | 41.1 ± 0.1 | 5.9 ± 0.1 | 39.9 ± 0.3 | 11.5 ± 0.4 | 6.4 ± 0.3 | 20.8 ± 0.4 |
| B | 59.2 ± 0.4 | 8.0 ± 0.2 | 56.0 ± 0.2 | 15.2 ± 0.1 | 10.2 ± 0.2 | 38.3 ± 0.9 | |
| WW4 | A | 228.1 ± 1.9 | 11.6 ± 0.4 | 145.8 ± 0.6 | 52.8 ± 0.9 | 30.7 ± 0.8 | 124.5 ± 1.4 |
| B | 262.0 ± 1.7 | 16.2 ± 0.2 | 152.0 ± 1.1 | 52.9 ± 0.4 | 28.8 ± 0.4 | 162.2 ± 1.3 | |
| IPLA838 | A | 11.0 ± 0.3 | 3.9 ± 0.1 | 1.6 ± 0.1 | 1.5 ± 0.1 | 0.6 ± 0.1 | 2.0 ± 0.1 |
| B | 21.1 ± 0.4 | 6.3 ± 0.1 | 3.8 ± 0.2 | 3.2 ± 0.1 | 0.4 ± 0.2 | 2.8 ± 0.1 | |
| aa solution | 991.3 ± 1.5 | 144.4 ± 1.1 | 642.0 ± 2.6 | 211.8 ± 1.3 | 531.1 ± 0.3 | 790.0 ± 0.9 | |
Values indicate concentration (μM). Two sampling points are shown: A-end of lag growth phase, and B- mid exponential growth phase (see Fig. S4). After the strains were grown in CDM-casein medium, the concentrations of amino acids in the culture supernatants were measured using a HPLC assay coupled with fluorescence detection. Strains with positive and negative amino acid secretion capacity are shown. A positive (standard amino acid solution) and negative (CDM-casein) control are shown. Amino acids below the detection limit of the HPLC assay are indicated as ND. Quantifications were performed in duplicates, and average values are shown. Error is SD.