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. 2020 Jun 12;9(6):e1141. doi: 10.1002/cti2.1141

Figure 3.

Figure 3

DCs and naïve WT1‐specific CD8+ T cells develop in humanised mice. (a) Diagram showing the generation of humanised NSG‐A24 mice using cord blood‐derived HSCs transduced with a lentivirus expressing a WT1235–243‐specific TCR. (b, c) Human CD45+ cell reconstitution in spleens from humanised NSG‐A24 mice. (b) Representative dot plots. (c) Human CD45+ cell subset composition (Means ± SD from three mice). (d) Expression of the WT1235–243 ‐specific TCR on splenic human naïve CD3+ CD8+ T cells, detected using the corresponding tetramer and rat CD2. (e) Phenotype of CD3+ CD8+ T cells, gated on total transduced cells (rCD2+, top) or transduced cells expressing the WT1235–243‐specific TCR (bottom). CM, central memory (CD45RA− CCR7+); EM, effector memory (CD45RA− CCR7−); EMRA, terminally differentiated effector memory (CD45RA+ CCR7−); N, naïve (CD45RA+ CCR7+). (f) IFNγ production after incubation of humanised mouse splenocytes with WT1235–243 peptide (ELISPOT assay). Data represent pooled replicates from five humanised mice.