Table 2.

Contents of galactolipid species in 5 replicate samples of flours from the 7A:M and 7A:H sets of NILs grown in Trial 1.

Sample Replicates	MGDG 34:2	MGDG 36:3	MGDG 36:4	MGDG 36:5	DGDG 34:2	DGDG 34:3	DGDG 36:3	DGDG 36:4	DGDG 36:5
7A:M R1	3.51	5.02	88.29	11.43	34.93	8.06	12.3	141.85	37.07
7A:M R2	4.43	4.98	90.99	12.20	35.37	8.16	11.90	144.21	27.84
7A:M R3	6.92	7.68	222.88	29.47	44.98	10.91	14.60	188.54	38.99
7A:M R4	4.69	6.91	106.35	15.53	34.39	8.07	11.69	144.37	28.71
7A:M R5	5.11	7.98	118.89	16.93	43.99	11.32	15.58	151.10	38.57
Average	4.93	6.51	125.48	17.11	38.73	9.30	13.21	154.01	34.24
Standard Error	0.56	0.64	24.97	3.25	2.36	0.74	0.79	8.77	2.46
7A:H R1	6.54	15.13	230.81	31.43	44.80	11.13	16.32	183.67	39.90
7A:H R2	5.22	9.30	174.07	25.65	67.34	16.32	24.31	282.91	55.21
7A:H R3	7.62	11.34	170.70	25.29	58.09	12.99	19.58	233.14	51.00
7A:H R4	6.54	14.32	224.05	31.58	72.48	18.49	24.74	284.87	57.36
7A:H R5	6.81	18.66	242.61	33.20	76.20	17.77	23.87	306.10	63.41
Average	6.55	13.75	208.45	29.43	63.78	15.34	21.76	258.14	53.38
Standard Error	0.39	1.61	15.03	1.65	5.64	1.41	1.65	22.13	3.92
t-statistic	2.367	4.171	2.847	3.378	4.1	3.778	4.686	4.373	4.137
df	8	8	8	8	8	8	8	8	8
p-value	0.045	0.003	0.022	0.01	0.003	0.005	0.002	0.002	0.003

Nine species of galactolipid are quantified: four species of monogalactosyl diglyceride (MGDG 34:2, 36:3, 36:4 and 36:5) and five species of digalactosyl diglyceride (DGDG 34:2, 34:3, 36:3, 36:4 and 36:5).

Significance is assessed via a two-sample t-test. Where variances were deemed unequal, Satterthwaite’s approximation to the degrees of freedom was used to calculate Welch’s t-test.