. Author manuscript; available in PMC: 2020 Jun 13.

Published in final edited form as: Biochemistry. 2019 Apr 18;58(17):2269–2281. doi: 10.1021/acs.biochem.9b00123

Table 1.

Summary of the Sedimentation Parameters Determined from Velocity Centrifugation of Apo-S100A12

	233 ,μM S100A12^a	23 ,μM S100A12^a	6 μM S100A12^a	PDB entry 2WCF^b
S (20,w) (S)	2.195 (2.185, 2.205)	2.165 (2.163, 2.167)	2.483 (2.469, 2.497)	2.4
S/D (M_w) (kDa)	19.90 (19.25, 20.55)	20.75 (20.57, 20.93)	18.15 (17.11, 19.12)	20.3
D (20,w) (F)	10.53 (10.20, 10.86)	9.96 (9.87, 10.04)	13.05 (12.29, 13.89)	9.7

The parameters were obtained from fitting the time-derivative distributions shown in Figure 2 to a single-component model as described in Materials and Methods.

The hydrodynamic parameters were calculated from the indicated crystal structure using the program HydroPro as described in Materials and Methods.