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. Author manuscript; available in PMC: 2020 Jun 13.
Published in final edited form as: Biochemistry. 2019 Apr 18;58(17):2269–2281. doi: 10.1021/acs.biochem.9b00123

Table 2.

Summary of the Sedimentation Parameters Determined by Equilibrium Centrifugation of S100A12 Complexed with Ca2+, Zn2+, and Both Zn2+ and Ca2+

metal ion Mw (Da) Kd(DX-O) (μM)
20 mM Ca2+mM 32750 (28610, 37489) 4585.3 (4005.7, 5248.8)
0.2 mM Zn2+ 47320 (41005, 54607) 56.7 (49.1, 65.4)
0.2 mM Zn2+ and 20 Ca2+ 68225 (59228, 78588) 13.2 (11.5, 15.2)