Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 19;48(11):6001–6018. doi: 10.1093/nar/gkaa425

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 5. — ZKSCAN3 overexpression alleviates hMSC aging. (A) Immunostaining of Ki67 in RS (P10) (upper), LMNA^G608G/+ (P6) (middle) and an aged individual-derived primary (P8) (lower) hMSCs transduced with lentiviruses expressing Luc or ZKSCAN3, respectively. Scale bar, 25 μm. White arrows represent Ki67-positive cells. Data are presented as the mean ± SEMs, n = 3. *P < 0.05, **P < 0.01 (two tailed t-test). (B) Clonal expansion ability analysis of RS (P10) (upper), LMNA^G608G/+ (P6) (middle) and an aged individual-derived primary (P8) (lower) hMSCs transduced with lentiviruses expressing Luc or ZKSCAN3. Data are presented as the mean ± SEMs, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001 (two tailed t-test). (C) SA-β-gal staining of RS (P10) (upper), LMNA^G608G/+ (P6) (middle) and an aged individual-derived primary (P8) (lower) hMSCs transduced with lentiviruses expressing Luc or ZKSCAN3. Data are presented as the mean ± SEMs, n = 3. *P < 0.05, **P < 0.01 (two tailed t-test). (D) Relative nuclear size analysis in RS (P10) (upper), LMNA^G608G/+ (P6) (middle) and an aged individual-derived primary (P8) (lower) hMSCs transduced with lentiviruses expressing Luc or ZKSCAN3. Data are presented as the mean ± SEMs, n = 200. *P < 0.05, **P < 0.01 (two tailed t-test). (E) Heatmaps showing the mRNA expression levels of P16 and IL6 in RS (P10) (upper), LMNA^G608G/+ (P6) (middle) and an aged individual-derived primary (P8) (lower) hMSCs transduced with lentiviruses expressing Luc or ZKSCAN3 by RT-qPCR. (F) Immunostaining of HP1α in RS (P10) (left), LMNA^G608G/+ (P6) (middle) and an aged individual-derived primary (P8) (right) hMSCs transduced with lentiviruses expressing Luc or ZKSCAN3. Scale bar, 25 μm. Data are presented as the mean ± SEMs, n = 300. ***P < 0.001 (two tailed t-test). (G) Enrichment of H3K9me3 within the regions of repetitive sequences in RS (P10) (left), LMNA^G608G/+ (P6) (middle) and an aged individual-derived primary (P8) (right) hMSCs transduced with lentiviruses expressing Luc or ZKSCAN3 as measured by ChIP-qPCR. Data are presented as the mean ± SEMs. n = 4. **P < 0.01, ***P < 0.001 (two tailed t-test). (H) Heatmaps showing the mRNA expression levels of repetitive sequences in RS (P10) (left), LMNA^G608G/+ (P6) (middle) and an aged individual-derived primary (P8) (right) hMSCs transduced with lentiviruses expressing Luc or ZKSCAN3 by RT-qPCR.