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. 2020 May 6;48(11):6136–6148. doi: 10.1093/nar/gkaa297

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — The DXO family of proteins cleave RNA capped with nucleotide metabolites. Schematics of NAD, FAD, or dpCoA-capped RNA hydrolysis by the DXO family enzymes are shown on the right. ³²P-uniformly labeled RNAs (100 nM) capped with NAD, FAD or dpCoA as indicated were subjected to hydrolysis with mDXO (A), SpRai1 (B) or KlDxo1 (C) proteins in vitro. Data are derived from at least three independent experiments. The respective deNADding, deFADding and deCoAping products were resolved on 20% urea polyacrylamide gels without (C) or with 0.2% APB (A, B).