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. 2020 May 14;48(11):5986–6000. doi: 10.1093/nar/gkaa384

Figure 2.

Figure 2.

Loss of ZFX and ZNF711 in HEK293T cells inhibits cell proliferation. (A) Expression levels of ZFX/ZFY/ZNF711 in wt HEK293T cells. (B) Locations of gRNAs used to create CRISPR/Cas9-mediated ZFX and/or ZNF711 knockouts. The deletion of ZFX in ZFX KO clone1 and clone2 and the DKO clones were generated using ZFX gRNA1 and gRNA2. The deletion of ZNF711 in ZNF711 KO clone1 and the DKO clones was generated using ZNF711 gRNA1 and gRNA2; the deletion of ZNF711 KO clone2 was generated using ZNF711 gRNA2 and gRNA3. (C) Western blots showing the protein levels of ZFX and ZNF711 in wt HEK293T, ZFX KO clones, ZNF711 KO clones, and DKO clones; also shown is the level of p62 as a loading control. (D) Proliferation assays using wt HEK293T, two different ZFX and two different ZNF711 KO clones, and two DKO clones; data points are the mean of three biological replicates.