Figure 5.

ZFX and ZNF711 have properties of a transcription activator when bound downstream of the TSS. (A) ZFX and ZNF711 peak sets from HEK293T cells were clustered using K-means clustering, identifying four sets of peaks with distinct binding sites (left); cluster 4 (combination peaks) was subsequently re-clustered, identifying 4 subsets (right). Tag density plots for each of the 4 different clusters are presented on top of the heatmaps. (B) Average signals of ZFX and ZNF711 ChIP-seq reads in wt HEK293T at all promoters bound by each TF (top), promoters of genes with decreased expression in all three DKO clones (middle), and promoters of genes with increased expressions in all three DKO clones (bottom). Also shown, for both the downregulated and the upregulated gene categories, is the percentage of genes whose promoters are bound by ZFX or ZNF711 in peak categories 1–4, or not bound by ZFX or ZNF711.