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. 2020 May 14;48(11):5986–6000. doi: 10.1093/nar/gkaa384

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — ZFX ZF11-13 has very similar transcriptional activities as wt ZFX. (A) Volcano plots showing the differentially expressed genes (DEGs) identified via RNA-seq in comparisons of DKO cells 24 hr after transfection with FLAG-tagged wt ZFX vs. a control plasmid, FLAG-tagged ZFX ZF11-13 vs. a control plasmid, and FLAG-tagged wt ZFX vs. FLAG-tagged ZFX ZF11-13. (B) Shown is a Venn diagram comparing the 846 genes that are bound by ZFX and ZNF711 in wt HEK293T cells and show a decrease in mRNA levels in all three DKO clones and the 2275 genes that show increased levels in DKO cells transfected with either FLAG-tagged wt ZFX or ZFX ZF11-13. (C) Shown is a tag density plot of ChIP-seq data for endogenous ZFX, FLAG-tagged wt ZFX, ZFX ZF9-13, or ZFX ZF1-8 at the set of 277 responding promoters identified in panel B. (D) Motif coverage analysis of the 277 responding promoters.