Figure 1.
Mutation of pvrA reduces the virulence of PA14. (A). Bacterial colonization in the murine acute pneumonia model. Each mouse was infected intranasally with 4 × 106 CFU of wild type PA14, ΔpvrA or the complemented strain (ΔpvrA/pvrA). 12 hours post infection, mice were sacrificed and bacterial loads in the lungs were determined. Bars represent medians, and error bars represent standard deviations. *P < 0.05; ***P < 0.001 by Student's t-test. (B). Survival rates of infected mice. Mice were infected intranasally with 6 × 106 CFU of wild type PA14, the ΔpvrA mutant or the complemented strain (ΔpvrA/pvrA). The mice were monitored for 5 days. P values were calculated by the log-rank test.