Figure 10.
Role of the R136 in the function of PvrA. (A) The ΔpvrA mutant and the mutant carrying a wild type pvrA or the R136A mutant allele were grown in Glu-M9 and FA-M9. The relative mRNA levels of plcH, fadD1, fadD6 and PA0508 were determined by real time PCR. rpsL was used as the internal control. (B) Mice were infected intranasally with 4 × 106 CFU of the indicated strains. 12 hpi, the mice were sacrificed and bacterial loads in the lungs were determined by plating. Data represents the mean ± standard deviation from three samples. **P < 0.01, ***P < 0.001 by Student's t-test.